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IVF Treatment in Ahmedabad - Process, Eligibility & What to Expect

IVF (in vitro fertilisation) is a treatment in which eggs are retrieved from the ovaries, fertilised in an embryology laboratory, and the resulting embryos are transferred to the uterus. This guide explains the IVF process from initial assessment and ovarian stimulation through egg retrieval, fertilisation, embryo culture, embryo transfer and pregnancy testing.

IVF treatment is planned according to factors such as age, ovarian reserve, diagnosis, sperm parameters, previous treatment history and reproductive goals at our clinic. All controlled ovarian stimulation, egg retrieval, and blastocyst transfer procedures are performed under one roof at Wellspring IVF & Women’s Hospital — a leading IVF centre and fertility clinic in Ahmedabad (Level 2 ART Reg. No. GS/AHD/024). Every IVF cycle at our clinic is personally planned and performed by Dr. Pranay Shah, our senior IVF specialist and fertility doctor in Ahmedabad, ensuring single-doctor continuity of care. IVF is not automatically the first treatment for every couple; the appropriate approach depends on the clinical situation.

Starting IVF is one of the most significant decisions a couple will make. It deserves complete honesty — about what the process involves, what it costs, what success rate is realistic for your specific situation, and what to do if an IVF cycle fails.

This page answers everything. The complete IVF process — step by step. What IVF costs in Ahmedabad — with full transparency. What factors actually determine your success rate. And when IVF is — and is not — the right next step. If you have a question not answered here, the answer is one call away.

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What Is IVF? — In Vitro Fertilisation Explained Simply

IVF stands for In Vitro Fertilisation — ‘in vitro’ means ‘in glass’ in Latin, referring to the process happening outside the body in the embryology laboratory. In natural conception, fertilisation occurs inside the fallopian tube. In IVF, Dr. Shah’s team recreates this process with precision in our advanced lab — controlling conditions that nature cannot.

The process has five core phases: hormonal stimulation of the ovaries to produce multiple mature eggs, retrieval of those eggs under sedation, fertilisation with prepared sperm in the embryology lab, culture of the resulting embryo for 3–5 days, and transfer of the best-quality embryo to the uterus. The entire process, from stimulation to pregnancy test, takes approximately 4–6 weeks.

IVF is not a single ‘procedure’ — it is a precisely sequenced medical protocol where every decision — stimulation dose, trigger timing, sperm selection technique, transfer day, progesterone support — is individualised. The quality of these micro-decisions, made by the embryologist and the fertility consultant working in sync, is the true determinant of IVF success. This is where Wellspring IVF’s in-house team model delivers its advantage.


Advanced IVF Treatment in Ahmedabad at Wellspring IVF & Women’s Hospital showing IVF Process, embryo culture, fertilization, egg retrieval, and embryo transfer procedures

IVF at Wellspring — At a Glance

Feature / MetricDetails
What Is IVFIn Vitro Fertilisation — eggs retrieved from the ovaries, fertilised with sperm in our embryology lab, and the resulting embryo transferred to the uterus.
Typical Treatment Duration A fresh IVF cycle commonly spans approximately 4–6 weeks from stimulation through pregnancy testing.
IVF Cost Range₹99,999 to ₹2,50,000 per cycle (see our complete IVF cost breakdown). Cost depends on protocol complexity, medications, and add-ons required.
MonitoringFollicle growth and hormone levels are monitored during ovarian stimulation.
Fertilisation OptionsConventional IVF or ICSI may be used depending on semen parameters and clinical circumstances.
TechnologyAdvanced IVF lab with HEPA-filtered, VOC-controlled air systems. In-house embryology team. Blastocyst culture, vitrification, ICSI, IMSI, PGT-A.
Embryo CultureEmbryos may be cultured to Day 3 or Day 5/6 depending on the treatment plan and embryo development. We prefer Blastocyst culture in majority of cases where medically suitable.
Fresh vs FrozenBoth protocols available. Dr. Shah determines the optimal strategy at consultation — not a one-size-fits-all policy.
Pregnancy TestingBeta-hCG blood testing is performed after embryo transfer at the time advised by the treating team.
Step-by-Step Timeline

The IVF Process

DAY 1–2
Baseline

Initial Assessment & Baseline Scan

  • Transvaginal ultrasound — antral follicle count (AFC), uterine cavity assessment
  • Baseline blood tests — Day 2/3 FSH, LH, Estradiol, AMH confirmation
  • Protocol finalised — stimulation dose, medication schedule, calendar dates confirmed
  • Medication teaching — nurse explains injection technique (self-administration at home)
DAY 2–12
Stimulation

Controlled Ovarian Stimulation (COS)

  • FSH or hMG-based stimulation medication is prescribed according to the individual ovarian stimulation protocol.
  • GnRH antagonist added Day 5–6 to prevent premature ovulation
  • Monitoring scans every 2–3 days — follicle size, count, endometrial thickness
  • Blood tests — Estradiol (E2) levels guide dose adjustments in real time
  • Typical response: 8–15 mature follicles at Day 10–12 for good responders. The number of follicles and mature eggs varies considerably between patients and depends on age, ovarian reserve and response to stimulation.
  • Dr. Shah reviews every monitoring result personally before dose adjustment
DAY 12–14
Trigger

Trigger Injection — Final Egg Maturation

  • When follicular development and hormone levels indicate appropriate maturation, the final maturation trigger is administered at the timing determined by the treating team. (hCG or GnRH agonist)
  • Trigger type chosen based on OHSS risk — agonist trigger for high responders
  • Egg retrieval scheduled precisely 34–36 hours after trigger
  • Partner’s semen sample collection date and time confirmed
DAY 14
Retrieval

Egg Retrieval (Ovum Pick-Up)

  • Transvaginal ultrasound-guided follicle aspiration under IV sedation — 20–30 minutes
  • Performed by Dr. Shah personally — not delegated
  • Embryologist receives eggs immediately in adjacent lab for preparation
  • Recovery 2–4 hours. Discharge same day. Mild cramping is normal
  • Average eggs retrieved: 8–15 in good responders. Fewer in DOR patients — discussed beforehand
DAY 14–15
Fertilisation

Sperm Preparation & Fertilisation

  • Semen sample processed: density gradient + swim-up to select best sperm
  • ICSI treatment (recommended in most protocols): single best-quality sperm injected into each mature egg
  • Additional sperm-selection techniques may be considered in selected cases based on semen parameters, previous fertilisation outcomes and clinical assessment.
  • Fertilisation confirmed Day 1 (16–18 hours after ICSI): 2-pronucleate embryos counted
  • Fertilisation rate at Wellspring IVF: typically 70–80% of mature eggs
DAY 15–19
Culture

Embryo Culture — Day 1 to Day 5

  • Embryos cultured in time-lapse or standard incubators — controlled temperature, pH, gas, humidity
  • Daily assessment: cleavage rate, fragmentation, symmetry
  • Day 3 cleavage embryo: 6–8 cells — option for Day 3 transfer in selected cases
  • Day 5 blastocyst: inner cell mass + trophectoderm expansion — preferred transfer stage
  • PGT-A biopsy at Day 5 if chromosomal testing is part of the plan
DAY 19–20
Transfer

Embryo Transfer

  • Best-quality embryo selected — Gardner grading for blastocysts (aim: 4AA–5AA)
  • Soft catheter transfer under ultrasound guidance — no sedation needed, no pain
  • Single embryo transfer (eSET) is preferred to avoid twin risk
  • Luteal support begins: progesterone pessaries/gel (Crinone/Utrogestan) + oestrogen
  • Remaining good-quality embryos vitrified (frozen) for future FET cycles
  • Rest for 30 minutes post-transfer. Return to light activity same evening
DAY 31–33
Result

Pregnancy Test — Beta-hCG

  • Blood beta-hCG tested 14 days after transfer — the only reliable pregnancy confirmation
  • Positive result: progesterone support continued, first ultrasound at 6–7 weeks
  • Negative result: Dr. Shah consultation — review cycle, adjust protocol, plan next steps
  • Frozen embryos available for FET cycle — no repeat stimulation needed
  • Dr. Shah available by WhatsApp for questions during the 2-week wait

Want a detailed breakdown of what happens on every single day of your cycle? Read the complete IVF timeline, day by day to prepare for your treatment journey.

Fresh IVF Cycle vs Frozen Embryo Transfer (FET) — What Is the Difference?

One of the most common questions at consultation. The honest answer: it depends on your situation. Dr. Shah does not have a blanket policy — he has a clinical framework:

Factor

 Fresh IVF Cycle

Frozen Embryo Transfer (FET)

Embryo used

Created and transferred in same stimulation cycle

Embryo created earlier, frozen, thawed and transferred in a separate cycle

Endometrium

Stimulated (may not be optimal — raised progesterone can reduce receptivity)

Natural or medicated — endometrium fully prepared without stimulation interference

OHSS risk

Higher — especially in high responders

None — stimulation already completed

Best for

Good responders with normal progesterone on trigger day, 2–3 embryos only

High responders, OHSS risk, PGT-A cases, elevated progesterone at trigger, low-AMH freeze-all strategies

Success rates

Outcomes depend on patient characteristics, embryo quality, endometrial preparation and the reason a fresh or frozen transfer is selected. Neither approach is universally superior for every patient.

Equivalent or better in most patient groups — particularly for PGT-A cycles

Dr. Shah’s default

Fresh or frozen transfer is selected case by case based on ovarian response, hormone levels, embryo status, endometrial factors and whether embryo testing is planned.

Preferred for high responders and whenever progesterone rise >1.5 ng/mL on trigger day

Read full guide

 

→ FET Complete Guide

Watch Our IVF Treatment Video

Learn how IVF treatment works, when it may be recommended, and what couples can expect during the process.

What You Will Learn

  • IVF process step-by-step
  • Embryo transfer and IVF treatment basics
  • IVF success factors and common myths
  • When IVF may be recommended

IVF Cost: What Determines the Treatment Price?

The cost of IVF varies according to the treatment protocol, medication requirements and clinically indicated laboratory or genetic services. At Wellspring IVF & Women’s Hospital, the estimated cost is explained before treatment begins, with the components of the package and additional services discussed clearly during consultation.

compare Basic, Standard, and Advanced tiers in our IVF treatment cost and package guide for Ahmedabad. →
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What Our Patients Say

Real stories from real families who trusted us with their fertility journey
800+ Google Reviews  •  Verified Patient Testimonials
Ketan B. profile picture
Ketan B.
5 months ago
I visited many doctors before, but this doctor was the one who correctly identified my issue and provided the right treatment. I finally started seeing real results after consulting them. Very knowledgeable, attentive, and professional. Highly recommended.
vibha R. profile picture
vibha R.
6 months ago
Heartfelt thanks to the entire team of Wellspring Hospital. After feeling disappointed and losing hope at many places, coming here was the best decision.
A special thank you to Dr. Pranay Shah for his confidence, guidance, and the way he explained everything so patiently. His positive approach gave me so much strength, and today I am blessed with my baby.
Thank you to each and every member of the hospital for taking such great care of me and supporting me throughout this journey. Forever grateful. 💕
Kanal G. profile picture
Kanal G.
8 months ago
Some doctors treat symptoms. Rare ones treat the human being sitting in front of them.

He is, without a doubt, the most patient doctor I have ever met. Of course, treatment can be done by many. What truly sets him apart is his maturity, the way he pauses, explains, comforts, and most importantly, seeks your permission before moving forward. You never feel rushed. You never feel unheard. You feel respected.

And the staff deserves equal appreciation. They handle even the most anxious and impatient moments with such calm grace and dignity that you slowly find your own heartbeat settling down. It feels less like a clinic and more like a safe space.

I wholeheartedly recommend him to anyone who overthinks, seeks reassurance, or simply needs a doctor who believes comfort is the first step of healing. With him, care begins long before the treatment does.
Kul C. profile picture
Kul C.
9 months ago
Dr Shah is highly knowledgeable, through and dedicated. He explained every step of the process in simple terms, ensuring we were informed and comfortable. The entire team and staff are very kind and caring.
Highly recommend for their expertise, kindness and dedication. "Turned out dream into reality"
chandresh T. profile picture
chandresh T.
10 months ago
We had a great experience with Wellspring. Dr Pranay Shah is a very good person and possess the good knowledge. His guidance and treatment helped us fulfill our wishes. The hospital staff is also very kind and supportive. I strongly recommend Wellspring.
Ruchita S. profile picture
Ruchita S.
11 months ago
I want to express my heartfelt gratitude to Dr. Pranay Shah and the team at Wellspring IVF & Women’s Hospital. This journey is never easy, but Dr. Shah made me feel comfortable, cared for, and fully supported throughout the IVF process. Thank you
Mohamed I. profile picture
Mohamed I.
12 months ago
Our hearts are overflowing with gratitude and joy as we reflect on our incredible journey to parenthood, made possible by the extraordinary care and expertise of your team. The IVF process was, at times, daunting and exhausting, but your unwavering support, compassion, and professionalism helped us remain hopeful through every step. From the very first consultation to the celebratory moment when we learned our treatment was successful, we felt respected, understood, and truly cared for.Thank you for believing in us, never giving up, and guiding us through every challenge with warmth, patience, and encouragement. Your personalized guidance, gentle approach, and positive outlook gave us strength, and your medical skill brought our dream to life. We are forever grateful for your remarkable ability to merge empathy and science, giving hope to couples like us.
Our gratitude also extends to everyone in your clinic who offered a smile, reassurance, technical support, or a listening ear along the way. We feel incredibly blessed to have chosen your practice for our journey, and we will always cherish the precious gift you helped us receive.
Thank you, from the bottom of our hearts, for making our dream a reality.

800+ Positive Google Reviews

Read verified patient reviews on Google.

Speak With Our IVF Team

If you are evaluating IVF, donor egg IVF, fresh versus frozen transfer, or a Low AMH IVF Protocol, a fertility consultation can help determine the most appropriate next step.

IVF Success Rates at Wellspring IVF — What Actually Determines Your Chances?

Wellspring reports a 70%+ positive pregnancy outcome in a defined cohort of 500 women aged 25–35 who underwent transfer of a 4AA-grade blastocyst between January and November 2025. This figure represents that specific cohort and should not be interpreted as an overall IVF success rate across all patients or all treatment cycles. Individual outcomes vary according to age, ovarian reserve, diagnosis, sperm factors, embryo characteristics and other clinical factors.

Factor

Impact on Success Rate

Wellspring IVF Approach

Female Age

Most powerful predictor. Under 35: 65–75%. 35–37: 55–65%. 38–40: 35–50%. Over 40: 20–35% (own eggs)

Age-matched protocol. PGT-A offered for 38+ to select chromosomally normal embryos (learn how PGT-A improves IVF success).

Ovarian Reserve (AMH)

Low AMH reduces egg yield but does not preclude IVF. Quality matters more than quantity

Dr. Shah has a specific Low AMH IVF protocol. See: Low Ovarian Reserve guide by Dr.Pranay Shah

Uterine Cavity

Submucosal fibroids, polyps, septum, adhesions — all reduce implantation. Correctable

Hysteroscopy performed before IVF if cavity assessment shows any abnormality

Sperm Quality (DFI)

High DNA fragmentation reduces embryo quality and increases miscarriage risk despite good SA

DFI tested routinely in unexplained infertility + RPL. PICSI/TESE when elevated

Embryo Quality

Blastocyst quality (Gardner grade) is the strongest single predictor of implantation

Blastocyst culture standard. Day 5 transfer unless clinical indication for Day 3

Endometrial Receptivity

Thin endometrium (<7mm) or poor trilaminar pattern reduces implantation

ERA (Endometrial Receptivity Analysis) offered for recurrent implantation failure

Number of Embryos

More embryos = more transfer attempts. First cycle matters, but frozen embryos enable 2nd/3rd FET

Vitrification standard for all surplus good-quality embryos

Lifestyle Factors

Smoking reduces IVF success by 15–20%. BMI >30: reduced response

Pre-IVF lifestyle counselling. 90-day optimisation period recommended where timeline permits

A Note on Success Rate Claims:

Any clinic that quotes a single success rate number without qualification is presenting incomplete information. Key questions to ask: Is this per egg retrieval, per embryo transfer, or per couple? Does it include cancelled cycles? What age group does it reflect? What denominator is used? At Wellspring IVF, Dr. Shah provides your individualised probability at consultation — based on your specific age, AMH, diagnosis, and sperm parameters. That number is more useful than any clinic average.

When several embryo transfer procedures have not resulted in a pregnancy, the situation is reviewed as recurrent implantation failure before the same protocol is simply repeated

IVF Protocol Deep Dives — Choose Your Specific Treatment Guide

The IVF Hub gives you the complete overview. For the detailed, protocol-specific guide — what happens day by day in each type of cycle — choose the relevant child page below

Fresh IVF Cycle — Complete Guide

  • Full stimulation calendar — Day 1 to transfer
  • How Dr. Shah individualises stimulation dose and protocol type (long, short, antagonist)
  • What to expect during egg retrieval and the 2-week wait
  • Fresh transfer vs freeze-all decision — when Dr. Shah chooses each
  • Medications list — what you will be injecting and why

→  Read Fresh IVF Cycle complete guide:

Frozen Embryo Transfer (FET) — Complete Guide

  • Natural cycle FET vs medicated cycle — which is right for you
  • Endometrial preparation protocol — oestrogen and progesterone timing
  • Embryo thawing and quality assessment — the vitrification survival rate
  • The transfer procedure — softer, simpler, with no anaesthesia
  • FET success rates vs fresh cycle — the evidence clearly explained

→  Read Frozen Embryo Transfer(FET) complete guide:

Clinical Decision Note: Dr. Pranay Shah evaluates your hormone profile, trigger day progesterone, and OHSS risk before choosing your transfer type. Read our detailed clinical guide on when a fresh transfer beats a freeze-all (and vice-versa) to explore specific patient scenarios.

Your Fertility Consultant

IVF treatment at Wellspring is led by Dr. Pranay Shah, Director & Chief Fertility Consultant, with continuity of care across treatment planning, monitoring and follow-up. Dr. Pranay Shah’s profile →

Dr. Pranay Shah, MS (Obs & Gyn), Founder and Chief Fertility Consultant at Wellspring IVF Ahmedabad

Dr. Pranay Shah

Director & Chief Fertility Consultant
Divyesh Bhalodia Senior Embryologist at Wellspring IVF & Women’s Hospital Ahmedabad with more than 15 years of experience in IVF laboratory and embryo culture

Divyesh Bhalodia

Senior Embryologist
Urmi Chauhan embryologist at Wellspring IVF & Women’s Hospital Ahmedabad specializing in IVF laboratory and embryo culture procedures

Urmi Chauhan

Clinical Embryologist
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When Is IVF the Right Treatment? — Dr. Shah's Decision Framework

IVF is recommended when the clinical circumstances indicate that it offers an appropriate chance of achieving pregnancy compared with other available treatment options. The decision depends on the cause of infertility, duration of infertility, age, ovarian reserve, sperm factors, previous treatment and reproductive goals. 

Clear Indications for IVF

  • Blocked or damaged fallopian tubes: Both tubes blocked: IVF is the primary treatment — sperm cannot reach the egg naturally. One tube blocked with additional factors: IVF strongly preferred over IUI. Guide: Blocked Fallopian Tubes
  • Severe male factor infertility: Severe oligospermia, severe asthenospermia, azoospermia (with surgical sperm retrieval), high DFI — all direct IVF/ICSI indications where IUI is not adequate. Guide: Male Infertility Hub
  • Failed IUI (3+ cycles): After 3 failed IUI cycles with no explanatory cause found: escalate to IVF. Each additional IUI cycle beyond 3 has diminishing returns and unnecessarily delays the more effective treatment.
  • Severe endometriosis or adenomyosis: Advanced endometriosis affecting tubal function or ovarian reserve. Adenomyosis with recurrent implantation failure. IVF with possible FET + endometrial preparation.
  • Recurrent pregnancy loss with chromosomal cause: IVF with PGT-A to transfer only chromosomally normal embryos — the most effective treatment for chromosomally-driven RPL. Guide: Recurrent Miscarriage
  • Age-related fertility decline (35+): Women over 35 with 6+ months of unsuccessful attempts, or over 40 with any fertility concern: IVF gives the highest probability of pregnancy within the available time. IUI delays are not appropriate.
  • Unexplained infertility after thorough workup: If all investigations are normal and IUI has not succeeded: IVF allows observation of fertilisation and embryo development — which often reveals the cause (poor blastulation, high DFI) that was invisible to any pre-IVF test.
Get Detailed Cost Breakdown

When Another Fertility Treatment May Be More Appropriate

IVF is not the first treatment for every patient. Depending on the diagnosis, options such as ovulation induction or IUI may be considered before IVF. The choice should be based on the couple’s clinical findings and treatment history.

Explore IUI treatment guide.

What Supports IVF Care at Wellspring

In-house embryology team

At many IVF centres, the embryology lab is outsourced or shared. At our IVF Centre in Ahmedabad, our in-house embryology team works alongside the fertility consultant throughout fertilisation, embryo culture, assessment and cryopreservation.

Advanced IVF lab

Embryo culture is performed in a controlled laboratory environment with monitored temperature, gas and air-quality parameters.

Individualised treatment planning

Stimulation and transfer decisions are adapted to the patient’s age, ovarian reserve, diagnosis, response and treatment history. Wellspring treats couples from across the state — see all the areas we serve across Ahmedabad and Gujarat and how out-of-town treatment works.

Clear treatment and cost communication

Patients are informed about the rationale for recommended procedures, expected steps and relevant costs before treatment decisions are made.

“Every couple that comes to Wellspring IVF gets three things from me at the first meeting: a complete, honest assessment of their situation — no softening of difficult truths; a personalised treatment plan with a genuine rationale for every recommendation; and a full cost breakdown before they commit to anything. IVF is expensive. It is also emotionally demanding. The least I can do is make sure no couple is surprised — by a bill, by a protocol change, or by a result they were never prepared for. Transparency is not a feature of our practice. It is the foundation of it.”

— Dr. Pranay Shah, MS (ObGy), Director & Chief Fertility Consultant, Wellspring IVF & Women’s Hospital, Ahmedabad

Frequently Asked Questions

Common questions on IVF timing, success, fresh versus frozen transfer, and cycle planning.
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Dr. Pranay Shah's team is available 6 days a week. WhatsApp enquiries answered within the working day. First consultation is a conversation — not a commitment.